Product Specification :
Phage and plasmid DNA templates | Genomic DNA | cDNA | ||||
Target | 1-2 kb | 3-4 kb | 5-6 kb | 7-10 kb | Up to 2 kb | Up to 2 kb |
Denature | 20" 94 oC | 20" 94 oC | 20" 94 oC | 20" 94 oC | 15" 95oC | 20" 94oC |
Anneal | 2"(Tm-5) oC | 5"(Tm-5)oC | 30"(Tm-5) oC | 30"(Tm-5) oC | 30" (Tm-5)oC | 30"68oC |
Extend | 20"72oC | 40"72oC | 1-2" 72 oC | 4 -5" 72 oC | 30-60" 72oC | none |
Cycles | 25 | 25-30 | 30 | 30 | 30 | 30 |